Different strains of phosphate solubilizing bacteria psb were isolated from the rhizosphere of different plants of lahore district, pakistan. Phosphate solubilizing bacteria psb have the potential to enhance phosphate induced immobilization of metals to remediate contaminated soil. Phosphatesolubilizing bacteria play a vital role in p solubilization by producing organic acids. Phosphatesolubilizing bacteria are frequently used as plant growth promoters. Many bacteria and fungi are able to improve plant growth by solubilizing inorganic and organic phosphates in the soil 9,10. The principal mechanism for mineral phosphate solubilization is the production of organic acids, and acid. Phosphate solubilization by endophytic bacteria isolated. Bacteria are more effective in phosphorus solubilization than fungi alam et al. Among the whole microbial population in soil p, solubilizing bacteria comprise 150% and p solubilizing fungi 0. Isolation and identification of potential phosphate solubilizing bacteria from the rhizoplane of oryza sativa l. Rao and sinha 2 for detection of phosphatesolubilizing bacteria from soil. Usually, one gram of fertile soil contains 10 1 to 10 10 bacteria, and their live weight may exceed 2,000 kg. A study was conducted to isolate phosphate solubilizing bacteria psb from aerobic rice grown in penang malaysia and to determine some biochemical properties of the isolates such as, organic acids, enzymes, indoleacetic acid iaa, siderophore production and its antagonistic effect against pathogen rhizoctonia solani.
Pdf plant growth promotion by phosphate solubilizing. Spectrophotometric quantification of phosphate solubilization showed that all bacterial species and a. A number of heterotrophic microorganisms excreting organic acids which solubalized p that chelate cationic partners of p ions and release the p directly into solution he et al. These phosphate solubilizing bacteria psb are being used as biofertilizer since 1950s.
Phosphate solubilizing ability and phylogenetic diversity of bacteria from prich soils around dianchi lake drainage area of china. Inoculation of phosphate solubilizing micro organisms increase crop yield by 510 %. Phosphatesolubilizing bacteria psb function in soil phosphorus cycle, increasing the bioavailability of soil phosphorus for plants. Tofazzal islama,b, abhinandan deoraa, yasuyuki hashidokoa, atiqur rahmana, toshiaki itoa, and satoshi taharaa a graduate school of agriculture, hokkaido university, kitaku, sapporo 0608589, japan. Pseudomonas, bacillus, micrococcus, aspergillus, fusarium, etc.
The integrated use of rock phosphate and psm add 2030 kg p2o5ha. Plant growth promotion by phosphate solubilizing bacteria. Pdf phosphorous is one of the most abundant metallic element found in the earths crust and present in soils in both organic and inorganic. Several researchers have reported that inoculation with phosphate solubilizing bacteria result in improved growth, yield, and p uptake in several crops hameeda et al. Phosphatesolubilizing bacteria employ different strategies to convert unavailable forms of phosphate into available forms. Additionally, soils contain low molecular weight organic acids with one or more carboxylic groups and some of these acids like citrate, oxalate, acetate, malate, isocitrate, and tartrate. Phosphate solubilizing bacteria play a vital role in p solubilization by producing organic acids. For this purpose, 15 phosphorus solubilizing bacteria psb were isolated from wheat rhizospheric soils of peshawar and southern punjab region, pakistan. Occurrence, mechanisms and their role in crop production. Some reports dealing with the isolation and characterization of phosphate solubilizing bacteria are. Increasing the bioavailability of immobilized phosphorus p in soil by phosphatesolubilizing bacteria psb is an effective strategy for sustainable agronomic use of p and for mitigating the p crisis. Phosphate solubilizing bacteria and their role in plant growth promotion article pdf available in biotechnology advances 1745. Phosphate solubilizing bacteria are frequently used as plant growth promoters. Improving cadmium mobilization by phosphatesolubilizing.
Though some of the bacterial strain such as pseudomonads and bacilli. Pdf factors affecting phosphatesolubilizing activity of microbes. Isolation of phosphate solubilizing microorganism psms. Phosphate solubilizing microorganisms naturally occurring rhizospheric phosphorus solubilizing microorganism psm dates back to 1903 khan et al. Seed or soil inoculation with phosphate solubilizing bacteria is known to improve solubilization of fixed soil phosphorus and applied phosphates resulting in higher crop yields. Strains from the genera pseudomonas, bacillus and rhizobium are among the most powerful phosphate solubilizers. Several soil microorganisms, including bacteria and fungi, are able to mineralize organic phosphates and solubilize inorganic phosphates. A moderately halophilic bacterium was isolated from the sediment of daqiao saltern on the eastern coast of china, which also. Occurrence of phosphate solubilizing bacteria high proportion of psm is concentrated in the rhizosphere, and they are metabolically more active than from other sources. Number of microbial species plays key role in p solubilization these includes bacteria, fungi, actinomycetes and even algae. Psms are ubiquitous, and their figures differ from soil.
Soil is a natural basal media for microbial growth. The relationship between phosphate solubilizing bacteria psb and plants is synergistic in nature, as bacteria provide soluble phosphate and plants supply root borne carbon compounds mainly sugars that can be metabolized for bacterial growth perez et al. Nov 09, 2015 in soil phosphate solubilizing bacteria constitute 1 to 50%,while phosphate solubilizing fungi are 0. After nitrogen, phosphorus p is the second essential macronutrient for plant growth and development. The aim of this study was to isolate, characterize and use phosphate solubilizing bacteria to enhance the bioavailability of insoluble caphosphate for wheat plants. These potassium solubilizing bacteria ksb were found to dissolve potassium, silicon and aluminium from insoluble kbearing minerals such as micas, illite and orthoclases, by excreting organic acids which either directly dissolved rock k or chelated silicon ions to bring k into the solution14 16. Phosphatesolubilizing microorganisms associated with the. Phosphatesolubilizing bacteria after nitrogen, phosphorus p is the second essential macronutrient for plant growth and development. Initially, 50 isolates were isolated on the basis of zone of clearance around their. Inorganic phosphatesolubilizing bacteria ipb are an important microbial class in sediments, playing an essential role in the phosphorus cycle of. In this context, cellulolytic, nitrogenfixing nfixing, phosphatesolubilizing bacteria are very important functional groups in regulating the elemental cycle and plant nutrition, hence replenishing the nutrient. Please read the research publication on how phosphate solubilizing bacteria can reduce chemical inputs by at least 25%. Phosphatesolubilizing bacteriaassisted phytoremediation of. Characteristics of inorganic phosphatesolubilizing bacteria.
Isolation of phosphate solubilizing microorganism psms from. Utilization of phosphatesolubilizing bacteria as biofertilizers instead of synthetic chemicals is known to improve plant growth through the supply of plant nutrients, and may help to sustain environmental health and soil productivity. Isolation and characterization of phosphate solubilizing. Improving cadmium mobilization by phosphate solubilizing bacteria via regulating organic acids metabolism with potassium wanli li, junfeng wang, yao lv, haojie dong, lili wang, tao he, qusheng li guangdong key laboratory of environmental pollution and health, school of environment, jinan university, guangzhou, 510632, china. In the midst of the major soil degradation and erosion faced by tropical ecosystems, rehabilitated forests are being established to avoid the further deterioration of forest lands. Effect of coinoculation with phosphate and potassium. There are considerable populations of phosphate solubilizing bacteria in soil and in plant rhizosphere.
Application of potential phosphatesolubilizing bacteria. Isolation and molecular characterization of phosphate. Among the whole microbial population in soil, psb constitute 1 to 50 %, while phosphorus solubilizing fungi psf are only 0. Among the bacterial genera with this capacity are pseudomonas, bacillus, rhizobium, burkholderia, achromobacter, agrobacterium, microccocus, aereobacter, flavobacterium and erwinia. Request pdf mechanisms of phosphate solubilization by psb phosphate solubilizing bacteria in soil among the major nutrients, phosphorus is by far the least mobile and available to plants in.
Several researchers have reported that inoculation with phosphatesolubilizing bacteria result in improved growth, yield, and p uptake in several crops hameeda et al. The use of phosphate solubilizing bacteria as inoculants simultaneously increases p uptake by the plant and crop yield. Phosphate solubilizing bacteria after nitrogen, phosphorus p is the second essential macronutrient for plant growth and development. Phosphate solubilizing bacteria bioline international. However, there is a limit on the amount of phosphate which can be added to the environment due to the issue of eutrophication. Isolation and identification of phosphate solubilizing bacteria psb in the present study, the collected soil samples were evaluated in vitro for p solubilising bacteria in pikovskayas pkv plates supplemented with 1. Pdf phosphate solubilizing bacteria and their role in plant. The aim of this study was to isolate, characterize and use phosphate solubilizing bacteria to enhance the bioavailability of insoluble ca phosphate for wheat plants. Phosphate solubilizing bacteria psb have the potential to enhance phosphateinduced immobilization of metals to remediate contaminated soil. Tofazzal islama,b, abhinandan deoraa, yasuyuki hashidokoa, atiqur rahmana, toshiaki itoa, and satoshi taharaa a graduate school of agriculture, hokkaido university, kitaku, sapporo 0608589, japan b present address. These diazotrophic bacteria increased plant biomass up to 39 % and accumulation of phosphorus by 10%. Bano, rhizobium and phosphate solubilizing bacteria improve the yield and phosphorus uptake in wheat triticum aestivum l. Isolation and characterization of a phosphatesolubilizing. Isolation and characterization of phosphatesolubilizing.
Usually, one gram of fertile soil contains 10 1 to 10 10 bacteria, and their live weight may exceed 2,000 kg ha1. Jan 10, 2019 phosphate solubilizing ability and phylogenetic diversity of bacteria from prich soils around dianchi lake drainage area of china. In this regards phosphatesolubilizing microorganisms psm have been seen as best ecofriendly means for p nutrition of crop. The mineral nutrition of plants mainly depends on soil p content that can be assimilated as a soluble phosphate. Phosphate solubilizing bacteria employ different strategies to convert unavailable forms of phosphate into available forms.
Usually, one gram of fertile soil contains 101 to 1010 bacteria, and their live weight may exceed 2,000 kg. Phosphate solubilizing bacteria research papers academia. Pot1, can solubilize tricalcium phosphate ca 3 po 4 2 at a rate of 956 mg l. Pdf phosphate solubilizing bacteria and their role in.
Phosphatesolubilizing bacteria and fungi in various cultivated and virgin alberta soils. Phosphate solubilizing activity of some bacterial strains. The main purpose of this study was to identify new phosphate solubilizing bacteria isolated. Strains from the genera pseudomonas, bacillus, rhizobium, azospirillum, burkholderia, enterobacter, erwinia, serratia, alcaligenes, arthrobacter, acinetobacter and flavobacterium are among the most powerful phosphate. Characterization of diazotrophic phosphate solubilizing. Mechanisms of phosphate solubilization by psb phosphate.
The principal mechanism for mineral phosphate solubilization is the production of organic acids, and acid phosphatases play a major role in the mineralization of. Generally, substantial amount of phosphorus p occurs in soil ranging from 400 to 1,200 mgkg of soil, either in mineral forms, e. View phosphate solubilizing bacteria research papers on academia. Pdf phosphate solubilizing bacteria and their role in plant growth. Novel phosphatesolubilizing bacteria enhance soil phosphorus. Occurrence of phosphate solubilizing bacteria high proportion of psm is concentrated in the rhizosphere, and they are metabolically more active than from other sources vazquez et al.
The most efficient of the bacteria presented in this paper, pantoea sp. Isolation, screening and characterization of phosphate. Phosphate solubilizing bacteria and their role in plant. On the basis of the finding that psb were significantly more active than nonpsb in the presence of insoluble p, a cd raman band from active assimilation of d 2 oderived d was established as a biomarker for both inorganicphosphatesolubilizing bacteria and organicphosphatesolubilizing bacteria. They convert the nonavailable inorganic phosphate to its soluble form, thus increasing the availability of phosphorus by secreting organic acids like citric, oxalic, succinic, tartaric.
Phosphatesolubilizing bacteriaassisted phytoremediation. These isolates were identified using light microscopy and 16s rrna gene. Phosphate solubilizing microorganism plays an important role in the plant nutrition through increase in p uptake by the plants and their use as pgpr is an important contribution to biofertilization of agricultural crops. Microorganisms are integral in the natural phosphorus cycle. Assessment of their effect on growth promotion and phosphorous uptake of mung bean vigna radiata l. After 5 days, serial dilution of the enriched medium was carried out and. Mineral phosphate solubilization several reports have examined the ability of different bacterial species to solubilize insoluble inorganic phosphate compounds, such as tricalcium phosphate, dicalcium phosphate, hydroxyapatite, and rock phosphate 38. Application of potential phosphatesolubilizing bacteria and. Phosphate solubilizing bacteria from sunflower rhizosphere 439 centrifuged at 10,000 rpm for 15 minutes. Yeast extract in the medium provides nitrogen and other nutrients necessary to support bacterial growth. The use of phosphate solubilizing bacteria as inoculants simultaneously increases phosphorus uptake by the plant and crop yield. Isolation and characterization of two phosphatesolubilizing.
Mar 23, 2020 moreover, the strong correlations of the relative abundances of the five individual genome bins representing novel phosphate solubilizing bacteria with bioavailable soil p fig. Phosphate solubilizing biofertilizers as a component of inm many heterotropjic bacteria and fungi efficiently solubilize insoluble phosphate of soil and rock phosphates. This study found bacteria that solubilized phosphate at very high rates. The use of phosphate solubilizing microorganisms psms as biofertilizers for agriculture enhancement has been a subject of study for years. Hence, use of diazotrophic phosphatesolubilizing bacteria may represent an alternative technology for fertilization systems in maize plants. Fungi have been reported to possess greater ability to solubilize rp than bacteria 11.
Different strains of phosphatesolubilizing bacteria psb were isolated from the rhizosphere of different plants of lahore district, pakistan. Psb could convert these insoluble phosphates into available forms for plant via acidification, chelation, exchange reactions, and production of gluconic isolation and characterization of phosphatesolubilizing bacteria. Here, d2o isotope labeling combined with singlecell raman spectroscopy ramand2o was developed as an efficient activitybased approach to characterizing the presence and activity of psb in. They are considered as one of the key groups of soil micro flora involved in p cycling. Different salts and yeast extract supports the growth of organisms. Phosphate solubilizing activity is greater for fungi than bacteria. Moreover, the strong correlations of the relative abundances of the five individual genome bins representing novel phosphatesolubilizing bacteria with bioavailable soil p fig. Phosphate solubilizing microorganisms springerlink. To reduce the addition of mineral phosphorus to agricultural soils, research in naturally occurring phosphatesolubilizing microorganisms has been conducted for. Review open access phosphate solubilizing microbes. In soil phosphate solubilizing bacteria constitute 1 to 50%,while phosphate solubilizing fungi are 0.
Mostly, one gram of fertile soil contains 10 1 to 10 10 bacteria, and their live weight may exceed 2,000 kg ha. Responses to soil ph gradients of inorganic phosphate. Isolation of efficient phosphate solubilizing bacteria. Utilization of phosphate solubilizing bacteria as biofertilizers instead of synthetic chemicals is known to improve plant growth through the supply of plant nutrients, and may help to sustain environmental health and soil productivity. The colonies showing clear halo zones around the microbial growth were considered as phosphate solubilization. Qualitative estimation of all the suspected phosphate solubilizing bacteria were screened by inoculating and growing on pvk medium and nbrip medium with bromophenol blue. Phosphate solubilizing bacteria an overview sciencedirect. In addition it can also solubilise k, zn, fe and mn and is also found to have antagonistic activity against select phytopathogens viz. Isolation and application of salttolerant or halophilic psb will facilitate the development of salinealkali soilbased agriculture. Phosphate solubilizing bacteria psb function in soil phosphorus cycle, increasing the bioavailability of soil phosphorus for plants. A total of 37 phosphate solubilizing microbial colonies were isolated on the pikovskayas agar medium, containing insoluble tri calcium phosphate tcp from agricultural soil. Isolation and identification of potential phosphate. Wilczek buddhi charana walpola 1, and minho yoon1 the aim of this work was to isolate phosphate solubilizing bacteria psb and assess their effect on the growth of mung bean.
Several authors attribute the solubilization of inorganic insoluble phosphate by microorganisms to the production of organic acids and chelating oxo acids from sugars. Phosphate solubilizing microbes psms are a group of beneficial microorganisms capable of hydrolyzing organic and inorganic insoluble phosphorus. The objective of the study was to explore the capabilities of psb and evaluate their efficiency to enhance growth of sugarcane plants under greenhouse condition. Phosphate and potassium are major essential macronutrients for plant growth and development and soluble p and k fertilizers are commonly applied to re.
Gas chromatographic analyses of cellfree spent culture medium from the various bacteria. A study was conducted to isolate phosphatesolubilizing bacteria psb from aerobic rice grown in penang malaysia and to determine some biochemical properties of the isolates such as, organic acids, enzymes, indoleacetic acid iaa, siderophore production and its antagonistic effect against pathogen rhizoctonia solani. The aim of this work was to isolate phosphate solubilizing bacteria psb and assess their effect on the growth of mung bean. Pdf the use of phosphate solubilizing bacteria as inoculants simultaneously increases p uptake by the plant and crop yield. Diameter of halo zone was measured after 42 hours, up to 15 days.